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firefly luciferase reporter plasmids  (Genechem)

 
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    Genechem firefly luciferase reporter plasmids
    Firefly Luciferase Reporter Plasmids, supplied by Genechem, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/luciferase+reporter+plasmid/abcb11+construct+gv238+luciferase+promoter+reporter/pmc13254595-132-12-23
    Average 86 stars, based on 1 article reviews
    firefly luciferase reporter plasmids - by Bioz Stars, 2026-09
    86/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: METTL5 deficiency impairs osteogenesis through OSER1-dependent antioxidant regulation.
    Article Snippet: .. A dual-luciferase reporter plasmid was constructed by GeneChem by cloning the full-length sequence of mouse Oser1 downstream of the Firefly luciferase (Fluc) coding sequence, with Renilla luciferase (Rluc) included as an internal control. .. MC3T3-E1 cells were transfected with the reporter plasmids using Lipofectamine 3000 (L3000015, Invitrogen) according to the manufacturer’s instructions.

    Construct:

    Article Title: METTL5 deficiency impairs osteogenesis through OSER1-dependent antioxidant regulation.
    Article Snippet: .. A dual-luciferase reporter plasmid was constructed by GeneChem by cloning the full-length sequence of mouse Oser1 downstream of the Firefly luciferase (Fluc) coding sequence, with Renilla luciferase (Rluc) included as an internal control. .. MC3T3-E1 cells were transfected with the reporter plasmids using Lipofectamine 3000 (L3000015, Invitrogen) according to the manufacturer’s instructions.

    Cloning:

    Article Title: METTL5 deficiency impairs osteogenesis through OSER1-dependent antioxidant regulation.
    Article Snippet: .. A dual-luciferase reporter plasmid was constructed by GeneChem by cloning the full-length sequence of mouse Oser1 downstream of the Firefly luciferase (Fluc) coding sequence, with Renilla luciferase (Rluc) included as an internal control. .. MC3T3-E1 cells were transfected with the reporter plasmids using Lipofectamine 3000 (L3000015, Invitrogen) according to the manufacturer’s instructions.

    Sequencing:

    Article Title: METTL5 deficiency impairs osteogenesis through OSER1-dependent antioxidant regulation.
    Article Snippet: .. A dual-luciferase reporter plasmid was constructed by GeneChem by cloning the full-length sequence of mouse Oser1 downstream of the Firefly luciferase (Fluc) coding sequence, with Renilla luciferase (Rluc) included as an internal control. .. MC3T3-E1 cells were transfected with the reporter plasmids using Lipofectamine 3000 (L3000015, Invitrogen) according to the manufacturer’s instructions.

    Luciferase:

    Article Title: METTL5 deficiency impairs osteogenesis through OSER1-dependent antioxidant regulation.
    Article Snippet: .. A dual-luciferase reporter plasmid was constructed by GeneChem by cloning the full-length sequence of mouse Oser1 downstream of the Firefly luciferase (Fluc) coding sequence, with Renilla luciferase (Rluc) included as an internal control. .. MC3T3-E1 cells were transfected with the reporter plasmids using Lipofectamine 3000 (L3000015, Invitrogen) according to the manufacturer’s instructions.

    Control:

    Article Title: METTL5 deficiency impairs osteogenesis through OSER1-dependent antioxidant regulation.
    Article Snippet: .. A dual-luciferase reporter plasmid was constructed by GeneChem by cloning the full-length sequence of mouse Oser1 downstream of the Firefly luciferase (Fluc) coding sequence, with Renilla luciferase (Rluc) included as an internal control. .. MC3T3-E1 cells were transfected with the reporter plasmids using Lipofectamine 3000 (L3000015, Invitrogen) according to the manufacturer’s instructions.



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    Image Search Results


    ABLIM1 is a downstream target of miR-378a-5p and involves in abdominal aortic aneurysm development. (A) Volcano plot of differently expressed genes in GSE183464 and GSE237229 database. Venn diagram showed intersection of differentially expressed genes and predicted target genes including ABLIM1 , DDX5 and SLC7A1 . (B) RT-qPCR analysis of target genes Ablim1 , Ddx5 and Slc7a1 in the TNFα-treated vascular smooth muscle cells (n=3 per group). RT-qPCR analysis of Ablim1 , Ddx5 and Slc7a1 in the aortas of Ang II-treated mice (n=3 per group). (C) ABLIM1 expression in the aortas treated-with antagomir-NC or antagomir-378a-5p was identified using by immunofluorescence staining. ABLIM1 (red), α-SMA (green) and DAPI (blue). (D) Representative images of immunofluorescence staining identified ABLIM1 expression in aortas with angomir-NC or angomir-378a-5p. ABLIM1 (red), α-SMA (green) and DAPI (blue). (E) Conservatism analysis of the binding site for miR-378a-5p and ABLIM1 in in humans, mice and rat. (F) Luciferase activity in 293T cells transfected with mimics-miR-378a-5p together with ABLIM1 -3'UTR-wild type or mutant plasmid. Data are presented as the mean ± SEM. P-values were calculated by Student's t test (for B). ** P<0.01 vs. Control or saline or mimics-NC- ABLIM1 -3'UTR-WT. ABLIM1, actin-binding LIM protein 1; miR or miRNA, microRNA; RT-qPCR, reverse transcription-quantitative PCR; α-SMA, α-smooth muscle actin; NC, negative control; UTR, untranslated region; WT, wild-type; MUT, mutated.

    Journal: International Journal of Molecular Medicine

    Article Title: miRNA-378a-5p attenuates the development of abdominal aortic aneurysm via ABLIM1-MKL1 signaling pathways

    doi: 10.3892/ijmm.2026.5768

    Figure Lengend Snippet: ABLIM1 is a downstream target of miR-378a-5p and involves in abdominal aortic aneurysm development. (A) Volcano plot of differently expressed genes in GSE183464 and GSE237229 database. Venn diagram showed intersection of differentially expressed genes and predicted target genes including ABLIM1 , DDX5 and SLC7A1 . (B) RT-qPCR analysis of target genes Ablim1 , Ddx5 and Slc7a1 in the TNFα-treated vascular smooth muscle cells (n=3 per group). RT-qPCR analysis of Ablim1 , Ddx5 and Slc7a1 in the aortas of Ang II-treated mice (n=3 per group). (C) ABLIM1 expression in the aortas treated-with antagomir-NC or antagomir-378a-5p was identified using by immunofluorescence staining. ABLIM1 (red), α-SMA (green) and DAPI (blue). (D) Representative images of immunofluorescence staining identified ABLIM1 expression in aortas with angomir-NC or angomir-378a-5p. ABLIM1 (red), α-SMA (green) and DAPI (blue). (E) Conservatism analysis of the binding site for miR-378a-5p and ABLIM1 in in humans, mice and rat. (F) Luciferase activity in 293T cells transfected with mimics-miR-378a-5p together with ABLIM1 -3'UTR-wild type or mutant plasmid. Data are presented as the mean ± SEM. P-values were calculated by Student's t test (for B). ** P<0.01 vs. Control or saline or mimics-NC- ABLIM1 -3'UTR-WT. ABLIM1, actin-binding LIM protein 1; miR or miRNA, microRNA; RT-qPCR, reverse transcription-quantitative PCR; α-SMA, α-smooth muscle actin; NC, negative control; UTR, untranslated region; WT, wild-type; MUT, mutated.

    Article Snippet: The 3' untranslated region (3'UTR) of mouse Ablim1 , Ddx5 (Dead-box helicase 5), Slc7a1 (Cationic amino acid transporter 1) gene was amplified and cloned into the pMIR-REPORT Luciferase (OBiO Technology Company) to construct pMIR-REPORT Luciferase 3'UTR wild-type (WT) plasmids.

    Techniques: Quantitative RT-PCR, Expressing, Immunofluorescence, Staining, Binding Assay, Luciferase, Activity Assay, Transfection, Mutagenesis, Plasmid Preparation, Control, Saline, Reverse Transcription, Real-time Polymerase Chain Reaction, Negative Control